Raji Umar Aderogba 1, Gideon Godspromise Obinna 2 , Abdulhakeem Abdulsalam Oladipupo 3, Courage Humphrey Ojeilua 4, Ojo-Omoniyi Damilola Samuel 5, Hafsat Abubakar Garba 6
1 Department of Biotechnology, Poznan University of Life Sciences, Poznan, Poland
2Department of Pharmacy, Faculty of Science, Nnamdi Azikwe Uiversity Teaching Hospital, Anambra State.
3Department of Medicine and Surgery, College of Health Science, Usman Danfodiyo University, Sokoto, Nigeria
4Department of Plant Biology and Biotechnology , Faculty of Science, University of Benin, Nigeria
5 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Nigeria
6 Department of Chemistry, Faculty of Science, Nigeria Defence Academy, Nigeria
Abstract
The present study evaluates the total antioxidant capacity of the methanol extract of Terminalia avicennioides bark using the phosphomolybdenum assay as described by Prieto et al. (1999). The assay is based on the reduction of Mo(VI) to Mo(V) by the antioxidant compounds present in the extract, forming a green phosphate/Mo(V) complex with a maximum absorbance at 695 nm. A reaction mixture containing 20 µL of the extract and 200 µL of reagent solution (0.6 M sulfuric acid, 4 mM ammonium molybdate, and 28 mM sodium phosphate) was incubated at 95°C for 90 minutes in a microtitre plate. After cooling to room temperature, absorbance was measured spectrophotometrically at 695 nm. Ascorbic acid was used as the standard, and antioxidant activity was expressed as ascorbic acid equivalents (µg/mL). A blank solution consisting of the extract and phosphate buffer was used as a control. The results indicate that Terminalia avicennioides bark extract possesses significant antioxidant potential, supporting its traditional use in herbal medicine. This study contributes to the growing body of knowledge on natural antioxidants and their possible applications in pharmaceutical and nutraceutical formulations.
Keywords: Terminalia avicennioides, antioxidant activity, phosphomolybdenum assay, methanol extract, ascorbic acid equivalent, medicinal plant.
Publication History
Submitted: 4th of February, 2025
Accepted: 15th of March, 2025
Published: 27th of March, 2025
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Reference
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Citation
APA Style
Aderogba, R. U., Obinna, G. G., Abdulhakeem, A. O., Ojeilu, C. H., Ojo-Omoniyi, D. S., & Garba, H. A. (2025). Evaluation of the antioxidant activity of Terminalia avicennioides bark extract by the phosphomolybdenum method: Effects of extract form and concentration. International Journal of Scholarly Resources, 17(2).